Raw Data for BglB Variant A236V

Expression Data

SDS-PAGE
 1.546

Kinetic Assay Data

slope of A236V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.00E+00
B 25.00 0.0011 1.05E-03 1.20E-03
C 8.33 8.00E-04 8.50E-04
D 2.78 8.00E-04 9.00E-04 8.00E-04
E 0.93 4.00E-04 4.00E-04
F 0.31 3.00E-04 2.50E-04
G 0.10 3.00E-04 3.00E-04
H 0.03 0.00E+00 0.00E+00 0.00E+00
 1.546      100×

Protein purified on 10/24/2021 and assayed on 10/26/2021.
Data uploaded by jamdinh and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3042 0.2949 0.2858
B 25.00 0.2766 0.2821 0.2964
C 8.33 0.1958 0.2027 0.1592
D 2.78 0.1121 0.1206 0.1524
E 0.93 0.04915 0.04830 0.05810
F 0.31 0.01970 0.01795 0.02290
G 0.10 0.006900 0.007300 0.008600
H 0.03 0.003100 0.003000
 1.546      100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by WilliamBenfield and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of A236V A420 nm
T (°C) 1 2 3
A 50.0 1.10E-03 8.00E-04 1.80E-03
B 48.0 7.00E-04 8.00E-04 9.00E-04
C 46.0 5.00E-04 5.00E-04 6.00E-04
D 42.0 7.00E-04 8.00E-04 9.00E-04
E 38.0 3.30E-03 4.20E-03 6.20E-03
F 34.0 3.80E-03 3.80E-03
G 31.0 3.80E-03 4.00E-03 3.90E-03
H 30.0 3.90E-03 3.80E-03 3.60E-03

Protein purified on 11/4/2021 and assayed on 11/19/2021.
Data uploaded by jamdinh and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of A236V A420 nm
T (°C) 1 2 3
A 50.0 0.001200 0.001300
B 48.0 0.001400 0.001100
C 46.0 0.005200 0.003700
D 42.0 0.2682 0.2858
E 38.0 0.7912 1.175 1.422
F 34.0 1.520 1.533 1.458
G 31.0 1.662 1.639 1.649
H 30.0 1.761 1.707 1.718

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by WilliamBenfield and last updated on 2022-06-27 07:10:33.


Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.