Raw Data for BglB Variant Y333F

Expression Data

Yield (mg/mL): 2.65

Kinetic Assay Data

slope of Y333F A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.537	1.325	1.190
	B	25.00	1.380	1.378	1.354
	C	8.33	1.237	1.272	1.249
	D	2.78	0.8209	0.8785	0.8778
	E	0.93	0.4187	0.4102	0.4168
	F	0.31	0.1495	0.1460	0.1593
	G	0.10	0.06155	0.05790	0.06295
	H	0.03	0.0004000	0.0009000	0.0005500

Yield (mg/mL): 2.65     Dilution Factor:  10×

Download these data

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by Srihita and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of Y333F A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0009000	0.001000	0.0009000
	B	48.3	0.001000	0.001000	0.001100
	C	45.7	0.002000	0.001900
	D	42.4	0.07600	0.09320	0.09080
	E	37.7	0.9767	1.078	1.117
	F	33.6	1.322	1.325	1.418
	G	31.3	1.450	1.442	1.526
	H	30.0	1.612	1.630	1.601

Download these data

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by Srihita and last updated on 2022-06-27 07:10:33.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.