Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.96

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.5403	0.5610	0.5446
	B	25.00	0.5036	0.5109	0.4649
	C	8.33	0.3866	0.3866	0.3604
	D	2.78	0.2796	0.2790	0.2534
	E	0.93	0.1473	0.1326	0.1282
	F	0.31	0.05265	0.04870	0.04480
	G	0.10	0.01970	0.01850	0.01385
	H	0.03	0.007100	0.007150	0.006650

Yield (mg/mL): 2.96     Dilution Factor:  100×

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by kuocarys and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0007000	0.0007500	0.0007000
	B	48.0	0.001150	0.001050	0.001300
	C	46.0	0.003000	0.003000	0.002950
	D	42.0	0.1639	0.1490	0.1773
	E	38.0	1.093	1.118	1.098
	F	34.0	1.264	1.260	1.276
	G	31.0	1.401	1.401	1.378
	H	30.0	1.496	1.500	1.497

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by kuocarys and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.