D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E177D

Expression Data

Yield (mg/mL): 1.97

Kinetic Assay Data

**slope of E177D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.189	0.2539	0.2306
B	25.00	0.1816	0.2018	0.1968
C	8.33	0.1273	0.1523	0.1541
D	2.78	0.071	0.09265	0.09275
E	0.93	0.03875	0.04085	0.03855
F	0.31	0.0145	0.016	0.01565
G	0.10	0.00575	0.006	0.00595
H	0.03	0.0002	0.00015	0

Yield (mg/mL): 1.97 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by slee4970 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3811	0.418	0.4703
B	25.00	0.4223	0.3995	0.3932
C	8.33	0.3207	0.2511	0.4261
D	2.78	0.156		0.1696
E	0.93	0.05135	0.07115	0.0936
F	0.31	0.0109	0.0249	0.0392
G	0.10	0.00295	0.01275	0.01795
H	0.03	0.00025	0.00015	0

Yield (mg/mL): 2.17 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by slee4970 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of E177D A_{420 nm}**
	T (°C)	1	2	3
A	50.0	0.0006000		0.0007000
B	48.3	0.0008000	0.001000	0.0008000
C	45.7	0.001000	0.001200	0.001200
D	42.4	0.0371	0.0614	0.0469
E	37.7		0.648	0.6552
F	33.6	0.6545	0.8467	0.8708
G	31.3	0.8108	0.9333	0.8106
H	30.0	0.9168	0.9985	0.9769

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by slee4970 and last updated on 2022-06-27 07:10:33.

Reference Data for WT

**slope of E177D A_{420 nm}**
	T (°C)	1	2	3
A	50.0	0.0008	0.001	0.0007
B	48.0	0.001	0.0008	0.001
C	46.0	0.002	0.002	0.0022
D	42.0	0.1373	0.1456	0.1652
E	38.0	1.149	1.084	1.096
F	34.0	1.506	1.445	1.462
G	31.0	1.403	1.288	1.414
H	30.0	1.462	1.586	1.429

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by mkazuma and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.