Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3811	0.418	0.4703
	B	25.00	0.4223	0.3995	0.3932
	C	8.33	0.3207	0.2511	0.4261
	D	2.78	0.156		0.1696
	E	0.93	0.05135	0.07115	0.0936
	F	0.31	0.0109	0.0249	0.0392
	G	0.10	0.00295	0.01275	0.01795
	H	0.03	0.00025	0.00015	0

Yield (mg/mL): 2.17     Dilution Factor:  100×

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Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by slee4970 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0008000	0.001000	0.0007000
	B	48.0	0.001000	0.0008000	0.001000
	C	46.0	0.002000	0.002000	0.002200
	D	42.0	0.1373	0.1456	0.1652
	E	38.0	1.149	1.084	1.096
	F	34.0	1.506	1.445	1.462
	G	31.0	1.403	1.288	1.414
	H	30.0	1.462	1.586	1.429

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by isimbre and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.