Raw Data for BglB Variant V79I

Expression Data

Yield (mg/mL): 3.48

Kinetic Assay Data

slope of V79I A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.5311	0.5339	0.5694
	B	25.00	0.4438	0.4439	0.4794
	C	8.33	0.3608	0.3451	0.3634
	D	2.78	0.2455	0.266	0.2678
	E	0.93	0.1332	0.1259	0.1313
	F	0.31	0.04665	0.04915	0.0505
	G	0.10	0.01565	0.01615	0.0152
	H	0.03	0.006251	0.0061	0.0063

Yield (mg/mL): 3.48     Dilution Factor:  100×

Download these data

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by anushak and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of V79I A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.00075	0.0008	0.0009
	B	48.3	0.00115	0.0013	0.00115
	C	45.7	0.0036	0.0038	0.004
	D	42.4	0.1753	0.1613	0.1797
	E	37.7	1.142	1.132	1.144
	F	33.6	1.294	1.292	1.255
	G	31.3	1.399	1.384	1.376
	H	30.0	1.505	1.506	1.487

Download these data

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by anushak and last updated on 2022-06-27 07:10:33.

Reference Data for WT

slope of V79I A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0007000	0.0007500	0.0007000
	B	48.0	0.001150	0.001050	0.001300
	C	46.0	0.003000	0.003000	0.002950
	D	42.0	0.1639	0.1490	0.1773
	E	38.0	1.093	1.118	1.098
	F	34.0	1.264	1.260	1.276
	G	31.0	1.401	1.401	1.378
	H	30.0	1.496	1.500	1.497

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by kuocarys and last updated on 2022-06-27 07:10:33.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.