D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T352P

Expression Data

Yield (mg/mL): 0.241

Kinetic Assay Data

**slope of T352P A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.01785	0.01655	0.01645
B	25.00	0.01355	0.01525	0.01525
C	8.33	0.01135	0.01185	0.01150
D	2.78	0.005900	0.006000	0.005750
E	0.93	0.002850	0.002600	0.002500
F	0.31	0.001400	0.001050	0.001500
G	0.10	0.0008500	0.0009500	0.0008001
H	0.03	0.0004000		0.0004500

Yield (mg/mL): 0.241 Dilution Factor: 100×

Protein purified on 11/24/2021 and assayed on 12/1/2021.
Data uploaded by alvinkim and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1640	0.1748	0.1671
B	25.00	0.1406	0.1395	0.1357
C	8.33	0.1013	0.1060	0.09950
D	2.78	0.05760	0.05880	0.05655
E	0.93	0.02395	0.02745	0.02400
F	0.31	0.007900	0.01085	0.01000
G	0.10	0.002850	0.004150	0.003400
H	0.03	0.001100	0.001500	0.001250

Yield (mg/mL): 1.727 Dilution Factor: 100×

Protein purified on 11/24/2021 and assayed on 12/1/2021.
Data uploaded by alvinkim and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.