D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H178N

Expression Data

Yield (mg/mL): 2.586

Kinetic Assay Data

**slope of H178N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.08605	0.07015	0.08165
B	25.00	0.08200	0.08529	0.07455
C	8.33	0.04615	0.04665	0.04840
D	2.78	0.02285	0.01790	0.02695
E	0.93	0.01045	0.007200	0.01420
F	0.31	0.003600	0.002550	0.004900
G	0.10	0.001200	0.0009500	0.001650
H	0.03	0.0003500	0	0.0002500

Yield (mg/mL): 2.586 Dilution Factor: 100×

Protein purified on 10/26/2021 and assayed on 11/18/2021.
Data uploaded by yesminezahra and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2874	0.2849	0.2899
B	25.00	0.2444	0.2390	0.2612
C	8.33	0.2535	0.2749	0.2170
D	2.78	0.1428	0.1467	0.1190
E	0.93	0.06931	0.06606	0.04785
F	0.31	0.02285	0.02330
G	0.10	0.007200	0.007450
H	0.03	0.0002000	0.0002000

Yield (mg/mL): 4.176 Dilution Factor: 100×

Protein purified on 10/26/2021 and assayed on 11/18/2021.
Data uploaded by yesminezahra and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.