D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H223A

Expression Data

Yield (mg/mL): 2.52

Kinetic Assay Data

**slope of H223A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1803	0.1803	0.1803
B	25.00	0.1841	0.1886	0.1784
C	8.33	0.1242	0.1097	0.1340
D	2.78	0.06700	0.06425	0.08565
E	0.93	0.03310	0.03145	0.03820
F	0.31	0.01195	0.01225	0.01500
G	0.10	0.004450	0.004550	0.005000
H	0.03	0.0002000	0.02005	0.006250

Yield (mg/mL): 2.52 Dilution Factor: 100×

Protein purified on 10/26/2021 and assayed on 11/18/2021.
Data uploaded by yesminezahra and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2874	0.2849	0.2899
B	25.00	0.2444	0.2390	0.2612
C	8.33	0.2535	0.2749	0.2170
D	2.78	0.1428	0.1467	0.1190
E	0.93	0.06931	0.06606	0.04785
F	0.31	0.02285	0.02330
G	0.10	0.007200	0.007450
H	0.03	0.0002000	0.0002000

Yield (mg/mL): 4.176 Dilution Factor: 100×

Protein purified on 10/26/2021 and assayed on 11/18/2021.
Data uploaded by yesminezahra and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.