Raw Data for BglB Variant Y333F

Expression Data

SDS-PAGE
 1.546

Kinetic Assay Data

slope of Y333F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3282 0.3115 0.3234
B 25.00 0.3356 0.2808 0.2658
C 8.33 0.2292 0.2319 0.2281
D 2.78 0.2329 0.2036 0.1977
E 0.93 0.1104 0.1083 0.1052
F 0.31 0.0491 0.0453 0.0418
G 0.10 0.0188 0.01775 0.01635
H 0.03 0.0008 0.00085 0.00105
 1.546      100×

Protein purified on 11/18/2021 and assayed on 11/26/2021.
Data uploaded by Langwin and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3394 0.3278 0.3284
B 25.00 0.2976 0.2995 0.3014
C 8.33 0.3544 0.2882 0.2696
D 2.78 0.2086 0.2075 0.2209
E 0.93 0.1125 0.1292 0.1217
F 0.31 0.05035 0.05100 0.04540
G 0.10 0.02075 0.02190 0.01860
H 0.03 0.003500 0.002800 0.002150
 2.24      100×

Protein purified on 11/18/21 and assayed on 11/18/21.
Data uploaded by thuang and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of Y333F A420 nm
T (°C) 1 2 3
A 50.0 0.0008 0.0008
B 48.3 0.0009 0.0008
C 45.7 0.0076 0.0092
D 42.4 0.2941 0.3397 0.312
E 37.7 1.291 1.114 1.345
F 33.6 1.673 1.643 1.596
G 31.3 1.601 1.538 1.485
H 30.0 1.742 1.924 1.93

Protein purified on 11/18/2021 and assayed on 11/18/2021.
Data uploaded by Langwin and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of Y333F A420 nm
T (°C) 1 2 3
A 50.0 0 0.0005000 0.0007000
B 48.3 0.0009000 0.001100 0.0009000
C 45.7 0.005300 0.006600
D 42.4 0.2606 0.3161 0.3037
E 37.7 0.8638 0.9226 1.137
F 33.6 1.653 1.533 1.388
G 31.3 1.134 1.256 1.339
H 30.0 1.440 1.890 1.644

Protein purified on 11/18/21 and assayed on 11/18/21.
Data uploaded by thuang and last updated on 2022-06-27 07:10:33.


Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.