D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D25Q

Expression Data

Yield (mg/mL): 0.638

Kinetic Assay Data

Plate kinetic-amylase

**slope of D25Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.06535	0.07995	0.07335
B	25.00	0.0682	0.06675	0.062
C	8.33	0.04725	0.05315	0.054
D	2.78	0.03065	0.02925	0.0276
E	0.93	0.0169	0.01545	0.0141
F	0.31	0.0061	0.00505	0.0051
G	0.10		0.0019	0.00185
H	0.03	0.00095	0.00085	0.00085

Yield (mg/mL): 0.638 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5403	0.561	0.5446
B	25.00	0.5036	0.5109	0.4649
C	8.33	0.3866	0.3866	0.3604
D	2.78	0.2796	0.279	0.2534
E	0.93	0.1473	0.1326	0.1282
F	0.31	0.05265	0.0487	0.0448
G	0.10	0.0197	0.0185	0.01385
H	0.03	0.0071	0.00715	0.00665

Yield (mg/mL): 2.96 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.