D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N160V

Expression Data

Yield (mg/mL): 1.85

Kinetic Assay Data

Plate kinetic-amylase

**slope of N160V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2503	0.2503	0.2655
B	25.00	0.1789	0.1929	0.1944
C	8.33	0.1072	0.12	0.1206
D	2.78		0.08135	0.09205
E	0.93	0.05365	0.0542	0.0548
F	0.31	0.0243	0.022	0.02205
G	0.10	0.0095	0.0072	0.00805
H	0.03	0.0039	0.00335	0.00355

Yield (mg/mL): 1.85 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5403	0.561	0.5446
B	25.00	0.5036	0.5109	0.4649
C	8.33	0.3866	0.3866	0.3604
D	2.78	0.2796	0.279	0.2534
E	0.93	0.1473	0.1326	0.1282
F	0.31	0.05265	0.0487	0.0448
G	0.10	0.0197	0.0185	0.01385
H	0.03	0.0071	0.00715	0.00665

Yield (mg/mL): 2.96 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of N160V A_{420 nm}**
	T (°C)	1	2	3
A	50.0	0.0009	0.0011	0.0008
B	47.1	0.0013	0.0012	0.0009
C	44.3	0.0031	0.0032	0.003
D	41.4	0.1622	0.1453	0.1697
E	38.6	1.213	1.228	1.299
F	35.7	1.246	1.402
G	32.9	1.454	1.501	1.422
H	30.0	1.529	1.633	1.575

Protein purified on 11/11/21 and assayed on 11/11/21.
Data uploaded by vater and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.