D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant V79I

Expression Data

Yield (mg/mL): 3.48

Kinetic Assay Data

Plate kinetic-amylase

**slope of V79I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5311	0.5339	0.5694
B	25.00	0.4438	0.4439	0.4794
C	8.33	0.3608	0.3451	0.3634
D	2.78	0.2455	0.266	0.2678
E	0.93	0.1332	0.1259	0.1313
F	0.31	0.04665	0.04915	0.0505
G	0.10	0.01565	0.01615	0.0152
H	0.03	0.006251	0.0061	0.0063

Yield (mg/mL): 3.48 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5403	0.561	0.5446
B	25.00	0.5036	0.5109	0.4649
C	8.33	0.3866	0.3866	0.3604
D	2.78	0.2796	0.279	0.2534
E	0.93	0.1473	0.1326	0.1282
F	0.31	0.05265	0.0487	0.0448
G	0.10	0.0197	0.0185	0.01385
H	0.03	0.0071	0.00715	0.00665

Yield (mg/mL): 2.96 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.