D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A236V

Expression Data

Yield (mg/mL): 0.504

Kinetic Assay Data

Plate kinetic-digestase2

**slope of A236V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0	0.0004
B	25.00	0.0011	0.00105	0.0012
C	8.33		0.0008	0.00085
D	2.78	0.0008	0.0009	0.0008
E	0.93	0.0004	0.0004
F	0.31	0.0003	0.00025	0.0004
G	0.10		0.0003	0.0003
H	0.03	0	0	0

Yield (mg/mL): 0.504 Dilution Factor: 10×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3042	0.2949	0.2858
B	25.00	0.2766	0.2821	0.2964
C	8.33	0.1958	0.2027	0.1592
D	2.78	0.1121	0.1206	0.1524
E	0.93	0.04915	0.0483	0.0581
F	0.31	0.0197	0.01795	0.0229
G	0.10	0.0069	0.0073	0.0086
H	0.03	0.0031	0.003

Yield (mg/mL): 1.546 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.