D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y333F

Expression Data

Yield (mg/mL): 2.65

Kinetic Assay Data

Plate kinetic-lactase2

**slope of Y333F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.537	1.325	1.19
B	25.00	1.38	1.378	1.354
C	8.33	1.237	1.272	1.249
D	2.78	0.8209	0.8785	0.8778
E	0.93	0.4187	0.4102	0.4168
F	0.31	0.1495	0.146	0.1593
G	0.10	0.06155	0.0579	0.06295
H	0.03	0.0004	0.0009	0.00055

Yield (mg/mL): 2.65 Dilution Factor: 10×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3029	0.3025	0.3264
B	25.00	0.2639	0.267	0.297
C	8.33	0.2189	0.2117	0.295
D	2.78	0.1575	0.1526	0.1557
E	0.93	0.0765	0.06545	0.08315
F	0.31	0.03285	0.02575	0.0321
G	0.10	0.0103	0.0093	0.0121
H	0.03	0.00055	0.00025	0.00085

Yield (mg/mL): 2.23 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.