D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N160V

Expression Data

Yield (mg/mL): 2.69

Kinetic Assay Data

Plate kinetic-lactase2

**slope of N160V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.09155	0.123	0.074
B	25.00	0.07105	0.08775	0.1077
C	8.33	0.06595	0.05775	0.0541
D	2.78		0.05005	0.0476
E	0.93	0.02765	0.01745	0.0316
F	0.31		0.01805	0.01825
G	0.10	0.0053	0.00315	0.00615
H	0.03	0.0003	0.0005	0.00035

Yield (mg/mL): 2.69 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3029	0.3025	0.3264
B	25.00	0.2639	0.267	0.297
C	8.33	0.2189	0.2117	0.295
D	2.78	0.1575	0.1526	0.1557
E	0.93	0.0765	0.06545	0.08315
F	0.31	0.03285	0.02575	0.0321
G	0.10	0.0103	0.0093	0.0121
H	0.03	0.00055	0.00025	0.00085

Yield (mg/mL): 2.23 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.