D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220F

Expression Data

Yield (mg/mL): 2.12

Kinetic Assay Data

Plate kinetic-lactase2

**slope of N220F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2178	0.2164	0.208
B	25.00	0.1958	0.2026	0.1982
C	8.33	0.1989	0.2045	0.1887
D	2.78	0.1679	0.1557	0.172
E	0.93	0.1281	0.1272	0.1479
F	0.31	0.0708	0.0869	0.08805
G	0.10	0.03215	0.03135	0.0359
H	0.03		0.0005	0.00055

Yield (mg/mL): 2.12 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3029	0.3025	0.3264
B	25.00	0.2639	0.267	0.297
C	8.33	0.2189	0.2117	0.295
D	2.78	0.1575	0.1526	0.1557
E	0.93	0.0765	0.06545	0.08315
F	0.31	0.03285	0.02575	0.0321
G	0.10	0.0103	0.0093	0.0121
H	0.03	0.00055	0.00025	0.00085

Yield (mg/mL): 2.23 Dilution Factor: 100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.