D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404D

Expression Data

Yield (mg/mL): 1.189

Kinetic Assay Data

**slope of N404D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.03760	0.04010	0.04170
B	25.00	0.02280	0.02190	0.02070
C	8.33	0.009800	0.01000	0.009900
D	2.78	0.003200	0.003600	0.003000
E	0.93	0.001600	0.001500	0.001600
F	0.31	0.0006000	0.0006000	0.0008000
G	0.10	0.0003000	0.0005000	0.0006000
H	0.03		0.0004000	0.0004000

Yield (mg/mL): 1.189 Dilution Factor: 10×

Protein purified on 11/17/21 and assayed on 11/18/21.
Data uploaded by anarsam and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4117	0.4200	0.4141
B	25.00	0.3291	0.3432	0.3228
C	8.33	0.2539	0.2557	0.2429
D	2.78	0.1056	0.1709	0.1528
E	0.93	0.03811	0.05600	0.05390
F	0.31	0.01240	0.02110	0.02800
G	0.10		0.006400	0.006400
H	0.03	0.0003000	0.0004000	0

Yield (mg/mL): 3.591 Dilution Factor: 100×

Protein purified on 11/17/21 and assayed on 11/18/21.
Data uploaded by anarsam and last updated on 2022-06-27 07:04:03.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.