D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T116I

Expression Data

Yield (mg/mL): 3.418

Kinetic Assay Data

**slope of T116I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1883	0.1937	0.1847
B	25.00	0.1289	0.1215	0.1310
C	8.33	0.05425	0.04820
D	2.78	0.03260	0.02435	0.006400
E	0.93	0.01380	0.009200	0.002550
F	0.31	0.005050	0.003800	0.001500
G	0.10	0.002300	0.001250	0.0008000
H	0.03	0.0006501		0.0009501

Yield (mg/mL): 3.418 Dilution Factor: 100×

Protein purified on 2/28/2022 and assayed on 3/2/2022.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2764	0.2756	0.2734
B	25.00	0.2308	0.2895	0.2651
C	8.33	0.1917	0.2001	0.2177
D	2.78	0.1255	0.1333	0.1307
E	0.93	0.08420	0.08451	0.05905
F	0.31	0.04300	0.04270
G	0.10	0.01750	0.01700	0.01360
H	0.03	0.001100		0.0007001

Yield (mg/mL): 4.166 Dilution Factor: 100×

Protein purified on 2/28/2022 and assayed on 3/1/2022.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:10:33.

Design Data

Rosetta Score for T116I: -7.27

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.