D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220C

Expression Data

Yield (mg/mL): 1.39

Kinetic Assay Data

**slope of N220C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.09900	0.1568	0.1359
B	25.00	0.1509	0.1503	0.1469
C	8.33	0.1749	0.1336	0.1549
D	2.78	0.1201	0.1101	0.09670
E	0.93	0.03840	0.02685	0.02970
F	0.31		0.01100	0.01000
G	0.10	0.005350	0.004350	0.003300
H	0.03	0.0000000	0.0005500	0.0002500

Yield (mg/mL): 1.39 Dilution Factor: 100×

Protein purified on 3/4/22 and assayed on 3/7/22.
Data uploaded by cnvrodriguez and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2437	0.1586	0.1933
B	25.00	0.2093	0.1905	0.1875
C	8.33	0.1578	0.1440	0.1238
D	2.78	0.06835	0.06395
E	0.93	0.03675	0.03125	0.03855
F	0.31	0.01085	0.01415	0.01555
G	0.10	0.003750	0.004900	0.005350
H	0.03		0.0002000	0.0002000

Yield (mg/mL): 2.3 Dilution Factor: 100×

Protein purified on 3/4/22 and assayed on 3/7/22.
Data uploaded by cnvrodriguez and last updated on 2022-06-27 07:04:03.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.