Raw Data for BglB Variant I91C

Expression Data

SDS-PAGE
 5.451

Kinetic Assay Data

slope of I91C A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.319 0.2947
B 25.00 0.2721 0.2715 0.2719
C 8.33 0.225 0.2193 0.2234
D 2.78 0.128 0.1417 0.1476
E 0.93 0.06315 0.0654
F 0.31 0.0246 0.02475 0.0243
G 0.10 0.0096 0.00945 0.0111
H 0.03 0 0 0
 5.451      10×

Protein purified on 3/25/22 and assayed on 4/1/22.
Data uploaded by lgara007 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.23E-02 9.06E-02 9.47E-02
B 25.00 7.86E-02 7.88E-02 7.98E-02
C 8.33 7.07E-02 7.04E-02 6.66E-02
D 2.78 6.69E-02 4.83E-02 7.08E-02
E 0.93 2.36E-02 2.22E-02 2.37E-02
F 0.31 9.00E-03 9.15E-03 9.30E-03
G 0.10 3.90E-03 3.45E-03 3.75E-03
H 0.03 6.00E-04 4.50E-04 4.50E-04
 2.032      100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by lalma020 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of I91C A420 nm
T (°C) 1 2 3
A 50.0 0 0
B 48.0 0.0015 0.0015
C 46.0 0.0018 0.0018
D 42.0 0.0129 0.0147 0.0126
E 38.0 1.073 1.034 0.9927
F 34.0 1.641 1.71 1.784
G 31.0 1.817 1.772 1.699
H 30.0 2 2.109 2.043

Protein purified on 3/25/22 and assayed on 4/1/22.
Data uploaded by lgara007 and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of I91C A420 nm
T (°C) 1 2 3
A 50.0 9.00E-04 1.20E-03
B 48.0 9.00E-04 1.20E-03
C 46.0 1.50E-03 1.50E-03 1.20E-03
D 42.0 3.63E-02 3.78E-02 3.63E-02
E 38.0 4.47E-01 4.43E-01 3.87E-01
F 34.0 4.43E-01 4.53E-01 4.54E-01
G 31.0 4.52E-01 4.81E-01 4.88E-01
H 30.0 4.81E-01 5.17E-01 5.03E-01

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by lalma020 and last updated on 2022-06-27 07:10:33.


Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.