Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.36

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.87E-02		7.40E-02
	B	25.00	6.84E-02		4.56E-02
	C	8.33	6.80E-02	5.37E-02	4.14E-02
	D	2.78	4.89E-02	4.07E-02	3.12E-02
	E	0.93		2.07E-02	1.80E-02
	F	0.31	1.37E-02	9.60E-03	7.95E-03
	G	0.10		3.15E-03	3.30E-03
	H	0.03	7.50E-04	7.50E-04

Yield (mg/mL): 1.36     Dilution Factor:  100×

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Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by carimaceo and last updated on 2023-09-11 10:28:44.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	50.0	1.50E-03	1.20E-03	1.20E-03
	B	48.3	9.00E-04		1.20E-03
	C	45.7	1.20E-03	1.20E-03	1.20E-03
	D	42.4	2.19E-02	2.34E-02
	E	37.7	4.00E-01	3.08E-01	4.01E-01
	F	33.6	3.56E-01	3.61E-01	3.46E-01
	G	31.3	3.66E-01	3.83E-01	3.72E-01
	H	30.0	3.76E-01	4.10E-01	3.71E-01

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Protein purified on 3/15/2022 and assayed on 4/1/2022.
Data uploaded by carimaceo and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.