Raw Data for BglB Variant K61M

Expression Data

SDS-PAGE
 4.601

Kinetic Assay Data

slope of K61M A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.72E-01 2.75E-01 2.79E-01
B 25.00 2.09E-01 2.08E-01 2.19E-01
C 8.33 1.74E-01 1.43E-01 1.83E-01
D 2.78 1.11E-01 1.05E-01 1.10E-01
E 0.93 5.78E-02 5.64E-02 5.37E-02
F 0.31 2.87E-02 2.96E-02 3.06E-02
G 0.10 1.11E-02 1.04E-02 1.07E-02
H 0.03 6.00E-04 7.50E-04
 4.601      100×

Protein purified on 3/25/22 and assayed on 4/1/22.
Data uploaded by mdiaz380 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.93E-01 3.35E-01
B 25.00 3.26E-01 3.34E-01
C 8.33 2.19E-01 1.78E-01 2.04E-01
D 2.78 1.48E-01 1.28E-01 1.48E-01
E 0.93 6.06E-02 5.24E-02 6.08E-02
F 0.31 1.38E-02 2.81E-02 2.39E-02
G 0.10 1.14E-02 1.22E-02
H 0.03 4.50E-04 6.00E-04 6.00E-04
 5.603      100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of K61M A420 nm
T (°C) 1 2 3
A 50.0 0 0
B 48.0 9.00E-04 1.20E-03
C 46.0 1.50E-03 1.50E-03
D 42.0 8.70E-03 7.80E-03 9.30E-03
E 38.0 7.41E-01 6.90E-01 6.88E-01
F 34.0 1.21E+00 1.24E+00 1.16E+00
G 31.0 1.20E+00 1.29E+00 1.39E+00
H 30.0 1.40E+00 1.35E+00 1.42E+00

Protein purified on 3/25/22 and assayed on 4/1/22.
Data uploaded by and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of K61M A420 nm
T (°C) 1 2 3
A 30.0 2.113 1.912 2.009
B 31.0 1.85E+00 1.78E+00 1.51E+00
C 34.0 1.28E+00 1.58E+00 1.71E+00
D 38.0 1.30E+00 1.08E+00 1.38E+00
E 42.0 4.86E-02 4.77E-02 4.44E-02
F 46.0 1.80E-03 0 2.70E-03
G 48.0 1.20E-03 1.20E-03 1.20E-03
H 50.0 1.50E-03 1.80E-03

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:10:33.


Design Data

2.61

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.