Raw Data for BglB Variant K61N
Expression Data
3.721
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 2.04E+00 | 2.01E+00 | 1.97E+00 | ||
| B | 25.00 | 1.58E+00 | 1.64E+00 | 1.56E+00 | ||
| C | 8.33 | 1.75E+00 | 1.32E+00 | 1.30E+00 | ||
| D | 2.78 | 9.01E-01 | 8.29E-01 | 6.72E-01 | ||
| E | 0.93 | 3.46E-01 | 3.15E-01 | 3.48E-01 | ||
| F | 0.31 | 1.37E-01 | 1.24E-01 | 1.30E-01 | ||
| G | 0.10 | 4.29E-02 | 4.47E-02 | 4.92E-02 | ||
| H | 0.03 | 0 | 1.20E-03 | 4.50E-04 |
Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by apena177 and last updated on 2022-06-27 07:04:03.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 3.02E-01 | 2.96E-01 | 2.99E-01 | ||
| B | 25.00 | 2.30E-01 | 2.39E-01 | 2.38E-01 | ||
| C | 8.33 | 1.76E-01 | 1.79E-01 | 2.08E-01 | ||
| D | 2.78 | 1.28E-01 | 1.27E-01 | 1.33E-01 | ||
| E | 0.93 | 6.66E-02 | 6.51E-02 | 7.64E-02 | ||
| F | 0.31 | 3.32E-02 | 3.17E-02 | 3.86E-02 | ||
| G | 0.10 | 1.25E-02 | 1.14E-02 | 1.64E-02 | ||
| H | 0.03 | 0 | 0 |
Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 0 | 0 | 0 | ||
| B | 48.3 | 0 | 0 | |||
| C | 45.7 | 0 | 0 | |||
| D | 42.4 | 4.20E-03 | 6.30E-03 | 4.80E-03 | ||
| E | 47.7 | 3.81E-01 | 1.71E-01 | 8.70E-03 | ||
| F | 33.6 | 7.37E-01 | 5.81E-01 | 2.82E-01 | ||
| G | 31.3 | 1.24E+00 | 1.47E+00 | 1.31E+00 | ||
| H | 30.0 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 30.0 | 2.078 | 1.876 | 1.811 | ||
| B | 31.0 | 1.58E+00 | 1.58E+00 | 1.50E+00 | ||
| C | 34.0 | 1.52E+00 | 1.56E+00 | 1.53E+00 | ||
| D | 38.0 | 1.37E+00 | 1.29E+00 | 1.20E+00 | ||
| E | 42.0 | 4.98E-02 | 4.92E-02 | 4.71E-02 | ||
| F | 46.0 | 2.10E-03 | 1.50E-03 | 1.80E-03 | ||
| G | 48.0 | 1.80E-03 | 1.50E-03 | 1.50E-03 | ||
| H | 50.0 | 1.50E-03 | 1.20E-03 | 1.50E-03 |
Protein purified on and assayed on .
Data uploaded by jnune033 and last updated on 2022-06-27 07:10:33.
Design Data
Citations
The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.