D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325R

Expression Data

Yield (mg/mL): 2.05

Kinetic Assay Data

**slope of W325R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.092	1.093	1.108
B	25.00	0.7796	0.7747	0.7698
C	8.33	0.6357	0.6320	0.6355
D	2.78	0.0004000	0.0005000
E	0.93	0.0004500	0.0006000	0.001000
F	0.31	0.0003500	0.0003000	0.0005000
G	0.10	0.0002000	0.0002500	0.0002500
H	0.03	0.0002500	0.0002500

Yield (mg/mL): 2.05 Dilution Factor: 10×

Protein purified on 2/24/2022 and assayed on 2/25/2022.
Data uploaded by bmagana1 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3435	0.3344	0.3088
B	25.00	0.2549	0.2855	0.2801
C	8.33	0.2361	0.2431	0.2529
D	2.78	0.1373	0.1505	0.1205
E	0.93	0.05565	0.07400	0.07160
F	0.31		0.02880	0.02640
G	0.10		0.01110	0.01120
H	0.03	0.002950	0.004350	0.003850

Yield (mg/mL): 3.334 Dilution Factor: 100×

Protein purified on 4/10/2022 and assayed on 4/11/2022.
Data uploaded by bmagana1 and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.