D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404M

Expression Data

Yield (mg/mL): 2.385

Kinetic Assay Data

**slope of N404M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3362	0.3453	0.3574
B	25.00	0.2453	0.2480	0.2367
C	8.33	0.1476	0.1595	0.1514
D	2.78	0.08826	0.09936	0.09313
E	0.93	0.06007	0.06394	0.06075
F	0.31	0.05074	0.05270	0.05009
G	0.10	0.04799	0.04768	0.04534
H	0.03		0.04290	0.04302

Yield (mg/mL): 2.385 Dilution Factor: 10×

Protein purified on 4/25/2022 and assayed on 4/26/2022.
Data uploaded by cgyeow and last updated on 2022-06-27 07:10:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.315	2.3010	2.287
B	25.00	2.016	2.113	2.122
C	8.33	1.524	1.718	1.579
D	2.78	0.9507	1.035	0.8190
E	0.93	0.3923	0.4791	0.3765
F	0.31	0.1834	0.2131	0.1749
G	0.10	0.1007	0.1065	0.08682
H	0.03	0.04170	0.03976	0.04271

Yield (mg/mL): 3.331 Dilution Factor: 100×

Protein purified on 4/25/2022 and assayed on 4/26/2022.
Data uploaded by cgyeow and last updated on 2024-06-07 13:14:56.

Design Data

Rosetta Score for N404M: -3.41

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.