D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W435Y

Expression Data

Yield (mg/mL): 0.484

Kinetic Assay Data

Plate kineticassay120Apr2022

**slope of W435Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1109	0.1133	0.1175
B	25.00	0.08065	0.08185	0.08345
C	8.33	0.06140	0.06375	0.06280
D	2.78	0.03390	0.03565	0.03705
E	0.93	0.01635	0.01745	0.01780
F	0.31	0.005650	0.005900	0.005950
G	0.10	0.002450	0.002200	0.002200
H	0.03	0.0001	0	0.0001500

Yield (mg/mL): 0.484 Dilution Factor: 100×

Protein purified on 18-04-22 and assayed on 20-04-22.
Data uploaded by srumjahn and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.9038	0.8951	0.9051
B	25.00	0.7615	0.7509	0.7514
C	8.33	0.6202	0.6205	0.6183
D	2.78	0.3792	0.3688	0.3681
E	0.93	0.1551	0.1556	0.1605
F	0.31	0.05670	0.05510	0.05650
G	0.10	0.02000	0.01865	0.01905
H	0.03	0.0002000	0	0.0006500

Yield (mg/mL): 4.552 Dilution Factor: 100×

Protein purified on 18-04-22 and assayed on 20-04-22.
Data uploaded by srumjahn and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.