D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409E

Expression Data

Yield (mg/mL): 0.782

Kinetic Assay Data

Plate kineticassay120Apr2022

**slope of W409E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	0.1475	0.1409	0.1480
C	8.33	0.1250	0.1369	0.1256
D	2.78	0.08760	0.08745	0.09365
E	0.93	0.04720	0.04760	0.04575
F	0.31	0.02095	0.02205	0.02290
G	0.10	0.006700	0.008150	0.008250
H	0.03	0		0

Yield (mg/mL): 0.782 Dilution Factor: 100×

Protein purified on 18-04-22 and assayed on 20-04-22.
Data uploaded by srumjahn and last updated on 2023-01-11 14:28:17.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.9038	0.8951	0.9051
B	25.00	0.7615	0.7509	0.7514
C	8.33	0.6202	0.6205	0.6183
D	2.78	0.3792	0.3688	0.3681
E	0.93	0.1551	0.1556	0.1605
F	0.31	0.05670	0.05510	0.05650
G	0.10	0.02000	0.01865	0.01905
H	0.03	0.0002000	0	0.0006500

Yield (mg/mL): 4.552 Dilution Factor: 100×

Protein purified on 18-04-22 and assayed on 20-04-22.
Data uploaded by srumjahn and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.