D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I244M

Expression Data

Yield (mg/mL): 2.972

Kinetic Assay Data

**slope of I244M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.58E-01	2.75E-01	2.44E-01
B	25.00	1.88E-01	1.95E-01	2.02E-01
C	8.33	1.74E-01	1.70E-01	1.67E-01
D	2.78	8.42E-02	8.13E-02	6.93E-02
E	0.93	6.50E-02	6.86E-02	5.36E-02
F	0.31	2.81E-02	4.74E-02	3.98E-02
G	0.10	1.43E-02	2.11E-02	1.91E-02
H	0.03	0	0

Yield (mg/mL): 2.972 Dilution Factor: 100×

Protein purified on 4/7/22 and assayed on 4/22/22.
Data uploaded by sydneysbecker and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.56E-01	2.47E-01	2.53E-01
B	25.00	2.16E-01	1.93E-01	2.03E-01
C	8.33	2.13E-01	1.79E-01	1.90E-01
D	2.78	1.09E-01	9.67E-02	9.80E-02
E	0.93	9.15E-02	8.57E-02
F	0.31	7.97E-02	7.42E-02
G	0.10	2.97E-02	2.40E-02	1.82E-02
H	0.03	3.00E-04	3.50E-04	3.50E-04

Yield (mg/mL): 3.308 Dilution Factor: 100×

Protein purified on 4/7/22 and assayed on 4/22/22.
Data uploaded by sydneysbecker and last updated on 2022-06-27 07:04:03.

Design Data

Rosetta Score for I244M: 6.85

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.