D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y166S

Expression Data

Yield (mg/mL): 1.644

Kinetic Assay Data

**slope of Y166S A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	2.16E-02	1.70E-02
B	25.00	1.69E-02	1.74E-02
C	8.33	1.03E-02	1.11E-02
D	2.78	8.10E-03	6.05E-03
E	0.93	2.40E-03	2.30E-03
F	0.31	1.25E-03	1.30E-03
G	0.10	5.00E-04	7.50E-04
H	0.03	4.00E-04	4.00E-04

Yield (mg/mL): 1.644 Dilution Factor: 100×

Protein purified on 4/7/22 and assayed on 4/22/22.
Data uploaded by sydneysbecker and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.56E-01	2.47E-01	2.53E-01
B	25.00	2.16E-01	1.93E-01	2.03E-01
C	8.33	2.13E-01	1.79E-01	1.90E-01
D	2.78	1.09E-01	9.67E-02	9.80E-02
E	0.93	9.15E-02	8.57E-02
F	0.31	7.97E-02	7.42E-02
G	0.10	2.97E-02	2.40E-02	1.82E-02
H	0.03	3.00E-04	3.50E-04	3.50E-04

Yield (mg/mL): 3.308 Dilution Factor: 100×

Protein purified on 4/7/22 and assayed on 4/22/22.
Data uploaded by sydneysbecker and last updated on 2022-06-27 07:04:03.

Design Data

Rosetta Score for Y166S: 8.17

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.