D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D35E

Expression Data

Yield (mg/mL): 1.999

Kinetic Assay Data

**slope of D35E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.68E-02	9.58E-02	1.05E-01
B	25.00	0.0804	0.07795	0.07825
C	8.33	0.07305	0.0652	0.0765
D	2.78	0.0527	0.05405	0.05785
E	0.93	0.02415	0.0201	0.01945
F	0.31	0.00765	0.0072	0.0077
G	0.10	0.003	0.00275	0.00205
H	0.03	0.00025	0.00025

Yield (mg/mL): 1.999 Dilution Factor: 10×

Protein purified on 5/9/22 and assayed on 5/12/22.
Data uploaded by clairehuntington and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.07E-01	8.71E-01	8.76E-01
B	25.00	7.51E-01	8.07E-01	8.13E-01
C	8.33	5.88E-01	6.61E-01	6.45E-01
D	2.78	4.07E-01	3.27E-01	3.94E-01
E	0.93	1.56E-01	1.23E-01	1.80E-01
F	0.31	6.61E-02	4.79E-02	7.30E-02
G	0.10	2.47E-02		2.52E-02
H	0.03	8.50E-04	6.00E-04	7.50E-04

Yield (mg/mL): 3.557 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by clairehuntington and last updated on 2023-11-07 18:27:52.

Design Data

Rosetta Score for D35E: 11.55

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.