Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2597	0.2567	0.2643
	B	25.00	0.219	0.1994	0.2278
	C	8.33	0.201	0.1943	0.1902
	D	2.78	0.0945	0.08905	0.08945
	E	0.93	0.0358	0.03195	0.034
	F	0.31	0.01235	0.00795	0.01105
	G	0.10	0.00505		0.0049
	H	0.03	0.0016	0.0012	0.0019

Yield (mg/mL): 3.25     Dilution Factor:  100×

Download these data

Protein purified on 5/19/22 and assayed on 5/26/22.
Data uploaded by mbettencourt and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.001		0.001
	B	48.3	0.0008		0.0008
	C	45.7	0.0013	0.0012	0.0014
	D	42.4	0.0884	0.119	0.1515
	E	37.7	0.8794	1.186	1.102
	F	33.6	1.458	1.369	1.344
	G	31.3	1.414	1.481	1.427
	H	30.0	1.439	1.235	1.188

Download these data

Protein purified on 5/19/22 and assayed on 5/26/22.
Data uploaded by mbettencourt and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.