D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D63M

Expression Data

Yield (mg/mL): 0.099

Kinetic Assay Data

**slope of D63M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	3
A	75.00		0
B	25.00	0	0
C	8.33	0	0
D	2.78	0.0004	0.00025
E	0.93	0
F	0.31		0
G	0.10	0
H	0.03	0	0

Yield (mg/mL): 0.099 Dilution Factor: 10×

Protein purified on 5/18/22 and assayed on 5/19/22.
Data uploaded by Sabrina and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2597	0.2567	0.2643
B	25.00	0.219	0.1994	0.2278
C	8.33	0.201	0.1943	0.1902
D	2.78	0.0945	0.08905	0.08945
E	0.93	0.0358	0.03195	0.034
F	0.31	0.01235	0.00795	0.01105
G	0.10	0.00505		0.0049
H	0.03	0.0016	0.0012	0.0019

Yield (mg/mL): 3.25 Dilution Factor: 100×

Protein purified on 3/18/2022 and assayed on 3/19/2022.
Data uploaded by and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of D63M A_{420 nm}**
	T (°C)	1	2	3
A	50.0	0.001		0.001
B	48.0	0.0008		0.0008
C	46.0	0.0013	0.0012	0.0014
D	42.0	0.0884	0.119	0.1515
E	38.0	0.8794	1.186	1.102
F	34.0	1.458	1.369	1.344
G	31.0	1.414	1.481	1.427
H	30.0	1.439	1.235	1.188

Protein purified on 5/18/2022 and assayed on 5/19/2022.
Data uploaded by sophiaharimoto and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.